![]() ![]() ![]() EMB2654 binds sequence specifically to this target sequence in vitro. Predictions of the EMB2654 binding site match a small RNA “footprint” located on the 5′ half of the trans-spliced intron that is almost absent in the partially complemented mutant. We show that EMB2654 is required for the trans-splicing of the plastid rps12 transcript and that therefore the emb2654 mutant lacks Rps12 protein and fails to assemble the small subunit of the plastid ribosome, explaining the loss of plastid translation and consequent embryo-lethal phenotype. We report the partial complementation and subsequent comparative molecular analysis of two nonviable mutants impaired in chloroplast translation, one ( emb2394) lacking the RPL6 protein, and the other ( emb2654) carrying a mutation in a gene encoding a P-class pentatricopeptide repeat protein.
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